Identifying Genes Linked to Biofilm Thickness in Streptococcus mutans
Author Information
Author(s): Shemesh Moshe, Tam Avshalom, Kott-Gutkowski Miriam, Feldman Mark, Steinberg Doron
Primary Institution: Hebrew University-Hadassah
Hypothesis
This study aims to identify differentially expressed genes associated with the thickness of S. mutans biofilms.
Conclusion
The study reveals that the expression of certain genes associated with biofilm thickness in S. mutans is not directly regulated by luxS and is not significantly influenced by sucrose.
Supporting Evidence
- 29 genes were differentially expressed in 400- vs. 100-micron biofilms.
- 39 genes were differentially expressed in 200- vs. 100-micron biofilms.
- 10 genes showed differential expression in both comparisons, all of which were upregulated.
Takeaway
This research found specific genes that help S. mutans bacteria form thicker biofilms, which are important for their survival and ability to cause dental problems.
Methodology
DNA-microarray analysis and real-time RT-PCR were used to identify gene expression differences in biofilms of varying thickness.
Limitations
The study primarily focuses on gene expression without exploring the functional implications of these genes in biofilm formation.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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