Immunomodulatory effects of recombinant BCG expressing pertussis toxin on TNF-alpha and IL-10 in a bladder cancer model
2008

Effects of a New Bladder Cancer Treatment

Sample size: 90 publication Evidence: moderate

Author Information

Author(s): Chade Daher C, Borra Ricardo C, Nascimento Ivan P, Villanova Fabiola E, Leite Luciana C, Andrade Enrico, Srougi Miguel, Ramos Kátia L, Andrade Priscila M

Primary Institution: University of São Paulo, São Paulo, Brazil

Hypothesis

Can recombinant BCG expressing pertussis toxin improve immune response and reduce tumor size in bladder cancer models?

Conclusion

The study found that rBCG-S1PT significantly reduced bladder tumor volume compared to BCG and PBS treatments.

Supporting Evidence

  • rBCG-S1PT treatment resulted in a significant reduction in bladder weight compared to BCG and PBS.
  • Increased levels of TNF-α were observed in both BCG and rBCG-S1PT treated groups.
  • Only the rBCG-S1PT group showed a significant increase in IL-10 levels compared to controls.

Takeaway

Researchers tested a new treatment for bladder cancer and found it worked better than the standard treatment.

Methodology

The study involved treating C57BL/6 mice with either BCG, rBCG-S1PT, or PBS and measuring bladder weight and cytokine levels after tumor implantation.

Limitations

The study was conducted in a preclinical model, which may not fully replicate human responses.

Participant Demographics

Ninety female C57BL/6 mice aged six to eight weeks.

Statistical Information

P-Value

p<0.001

Statistical Significance

p<0.001

Digital Object Identifier (DOI)

10.1186/1756-9966-27-78

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