Pollen Competition as a Reproductive Isolation Barrier Represses Transgene Flow between Compatible and Co-Flowering Citrus Genotypes
2011

Pollen Competition Limits Transgene Flow in Citrus

Sample size: 603 publication 10 minutes Evidence: high

Author Information

Author(s): Elsa Pons, Antonio Navarro, Patrick Ollitrault, Leandro Peña

Primary Institution: Centro de Protección Vegetal y Biotecnología, Instituto Valenciano de Investigaciones Agrarias (IVIA), Moncada, Valencia, Spain

Hypothesis

How does pollen competition affect transgene flow between genetically modified and non-modified citrus trees?

Conclusion

Pollen competition significantly reduces the flow of transgenes between genetically modified and non-modified citrus trees.

Supporting Evidence

  • The study showed low frequencies of transgene flow (0.17–2.86%) over seven years.
  • Paternity analyses indicated that non-GM pollen sources had higher mating success.
  • Mixed pollination experiments demonstrated that non-GM pollen outcompeted GM pollen.

Takeaway

This study found that when bees pollinate citrus trees, the pollen from non-GM trees is better at fertilizing flowers than pollen from GM trees, which helps keep GM traits from spreading.

Methodology

The study involved a seven-year field trial with different citrus genotypes to measure pollen-mediated transgene flow and assess the role of pollen competition.

Potential Biases

Potential bias due to the specific environmental conditions and genetic backgrounds of the citrus trees used.

Limitations

The study was limited to specific citrus genotypes and may not be generalizable to all fruit tree species.

Participant Demographics

The study involved various citrus genotypes, including transgenic and non-transgenic types.

Statistical Information

P-Value

p<0.05

Statistical Significance

p<0.05

Digital Object Identifier (DOI)

10.1371/journal.pone.0025810

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