Zeb1 and miR-200b in Mouse Inner Ear Morphogenesis
Author Information
Author(s): Hertzano Ronna, Elkon Ran, Kurima Kiyoto, Morrisson Annie, Chan Siaw-Lin, Sallin Michelle, Biedlingmaier Andrew, Darling Douglas S., Griffith Andrew J., Eisenman David J., Strome Scott E.
Primary Institution: University of Maryland, Baltimore
Hypothesis
The Zeb1/miR-200b pathway plays a key role in establishing epithelial and mesenchymal identity in the inner ear.
Conclusion
Misregulation of the ZEB1/miR-200b pathway in Twirler mice is associated with auditory and vestibular defects.
Supporting Evidence
- The study identified over 3,000 differentially expressed genes in the inner ear.
- Zeb1 and miR-200b were found to regulate epithelial and mesenchymal identities.
- Misregulation of the ZEB1 pathway was linked to malformations in Twirler mice.
- Cell type-specific expression patterns were used to identify candidate genes for deafness.
Takeaway
Scientists studied how certain genes help shape the inner ear in mice, finding that a specific gene pathway is important for its development.
Methodology
The study developed a protocol to sort inner ear tissues into major cellular components and performed transcriptome profiling.
Potential Biases
Potential bias in identifying epithelial markers due to the regulation of CD326 by Zeb1.
Limitations
The use of CD326 to separate epithelial from non-epithelial compartments may introduce bias.
Participant Demographics
Newborn wild-type and Twirler mutant mice were used in the study.
Statistical Information
P-Value
p=3.7*10−12 for cochlea; p=1.29*10−15 for vestibular system.
Statistical Significance
p<10−5
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website