Catalog of Neutral and Deleterious Polymorphism in Yeast
Author Information
Author(s): Scott W. Doniger, Hyun Seok Kim, Devjanee Swain, Daniella Corcuera, Morgan Williams, Shiaw-Pyng Yang, Justin C. Fay
Primary Institution: Washington University, St. Louis, Missouri, United States of America
Hypothesis
To identify deleterious and neutral DNA sequence variation within Saccharomyces cerevisiae.
Conclusion
The study found that a significant fraction of DNA sequence variations in yeast has deleterious effects on fitness.
Supporting Evidence
- 6% of the genome is variable among the studied yeast strains.
- 93% of identified polymorphisms are single nucleotide polymorphisms (SNPs).
- 12% of coding SNPs and 7% of noncoding SNPs are estimated to be deleterious.
Takeaway
Scientists looked at the DNA of different yeast strains to see which changes are harmful and which are not. They found many changes that could hurt the yeast's ability to survive.
Methodology
The genomes of two yeast strains were sequenced and compared to a reference genome to identify polymorphisms.
Potential Biases
Potential biases in identifying deleterious SNPs due to reliance on specific models and assumptions.
Limitations
The study may not account for all types of genetic variation due to the focus on specific strains.
Statistical Information
P-Value
p<1×10−299
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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