Production and Stability of Alpha Amylase from Aspergillus Species
Author Information
Author(s): Chimata Murali Krishna, Chetty Chellu S., Suresh Challa
Primary Institution: Acharya Nagarjuna University
Hypothesis
The study aimed to isolate a novel Aspergillus species capable of producing alpha amylase and to optimize its production and stability.
Conclusion
The optimized conditions led to a maximum alpha amylase production of 1734 U/mL, demonstrating the enzyme's potential for industrial applications.
Supporting Evidence
- The maximum enzyme production was achieved at a fermentor volume of 70%.
- The enzyme showed optimal activity at a temperature of 75°C and a pH of 5.0.
- Calcium chloride improved the thermostability of the enzyme, with the best results at 0.3 M concentration.
Takeaway
Scientists found a way to grow a special fungus that makes an important enzyme, which can help in cleaning cotton cloth by removing starch.
Methodology
The study involved isolating Aspergillus strains, optimizing fermentation conditions, and evaluating enzyme activity and stability.
Limitations
The study did not explore the long-term stability of the enzyme under various industrial conditions.
Digital Object Identifier (DOI)
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