Arp2/3 Complex Activity in Filopodia of Spreading Cells
Author Information
Author(s): Simon A Johnston, Jonathan P Bramble, Chun L Yeung, Paula M Mendes, Laura M Machesky
Primary Institution: University of Birmingham
Hypothesis
How does the Arp2/3 complex localize and function in filopodia during cell spreading?
Conclusion
Filopodia of spreading cells contain regions of lamellipodial activity, which influence their morphology and movement.
Supporting Evidence
- Arp2/3 complex localizes to filopodia and lamellipodia during cell spreading.
- About 30% of filopodia contained Arp2/3 complex at 15 and 30 minutes, increasing to 40% at 60 minutes.
- The presence of Arp2/3 complex in filopodia suggests a role in their dynamics and morphology.
Takeaway
Cells use tiny finger-like structures called filopodia to explore their surroundings, and a special protein complex helps them grow and move.
Methodology
Mouse embryonic fibroblasts were spread on fibronectin and analyzed for Arp2/3 complex localization using immunofluorescence and time-lapse microscopy.
Limitations
The study primarily focuses on mouse embryonic fibroblasts, which may not represent all cell types.
Participant Demographics
Mouse embryonic fibroblasts (MEFs) were used in the study.
Digital Object Identifier (DOI)
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