Effects of ECM Density on Integrin Clusters in Cells
Author Information
Author(s): Welf Erik S, Naik Ulhas P, Ogunnaike Babatunde A
Primary Institution: University of Delaware
Hypothesis
How does the concentration of extracellular matrix (ECM) affect integrin clustering in adherent cells?
Conclusion
Cells form smaller, less elongated integrin clusters that are closer to the cell periphery as ECM density increases.
Supporting Evidence
- Integrin cluster size decreases with increasing ECM concentration.
- Cells exhibit more total area occupied by bound integrins when adhering to surfaces coated with lower ECM.
- The distribution of integrin cluster sizes follows a lognormal distribution.
Takeaway
When cells stick to surfaces with more ECM, they make smaller and rounder clusters of proteins called integrins.
Methodology
Cells expressing integrin αIIbβ3 were seeded on different concentrations of fibrinogen, and integrin cluster properties were measured using immunofluorescence microscopy.
Limitations
The study only captures a single time point during dynamic processes of cell adhesion, spreading, and migration.
Digital Object Identifier (DOI)
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