Exportin 4 Interacts with Sox9 through the HMG Box and Inhibits the DNA Binding of Sox9
2011
Exp4 Regulates Sox9 Activity by Inhibiting Its DNA Binding
publication
Evidence: moderate
Author Information
Author(s): Tsuchiya Megumi, Ogawa Hidesato, Suzuki Taiga, Sugiyama Noriyuki, Haraguchi Tokuko, Hiraoka Yasushi
Primary Institution: Graduate School of Frontier Biosciences, Osaka University, Suita, Japan
Hypothesis
Does exportin 4 (Exp4) interact with Sox9 and regulate its transcriptional activity?
Conclusion
Exp4 acts as a co-regulator of Sox9 by inhibiting its ability to bind DNA and activate transcription.
Supporting Evidence
- Exp4 was identified as an interacting protein of Sox9 using affinity chromatography.
- Immunoprecipitation confirmed the interaction between Sox9 and Exp4 in human cells.
- Depletion of Exp4 increased transcription of Sox9 target genes.
- The HMG box of Sox9 was found to be responsible for binding to Exp4.
Takeaway
Exp4 is like a helper that stops Sox9 from sticking to the DNA, which means it can't tell the cell to make certain proteins.
Methodology
The study used affinity chromatography, immunoprecipitation, and chromatin immunoprecipitation to analyze the interaction between Exp4 and Sox9.
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website