Overexpression of cathepsin K in mice decreases collagen deposition and lung resistance in response to bleomycin-induced pulmonary fibrosis
2008

Cathepsin K Reduces Lung Fibrosis in Mice

Sample size: 10 publication Evidence: moderate

Author Information

Author(s): Mrigank Srivastava, Kathrin Steinwede, Riku Kiviranta, Jukka Morko, Heinz-Gerd Hoymann, Florian Länger, Frank Buhling, Tobias Welte, Ulrich A Maus

Primary Institution: Hannover School of Medicine, Hannover, Germany

Hypothesis

Over-expression of cathepsin K in the lungs of mice is protective in bleomycin-induced pulmonary fibrosis.

Conclusion

Over-expression of cathepsin K reduced lung collagen deposition and improved lung function parameters in the lungs of transgenic mice.

Supporting Evidence

  • Cathepsin K protein levels were significantly higher in transgenic mice compared to wild-type mice.
  • Transgenic mice showed lower collagen deposition in their lungs after bleomycin treatment.
  • Lung resistance was significantly lower in cathepsin K transgenic mice compared to wild-type mice after treatment.

Takeaway

Mice with extra cathepsin K in their lungs had less lung damage and better breathing after being treated with a harmful substance.

Methodology

Mice were treated with bleomycin and their lung function and collagen content were measured over several weeks.

Participant Demographics

Mice aged 8-12 weeks were used in the study.

Statistical Information

P-Value

p<0.05

Statistical Significance

p<0.05

Digital Object Identifier (DOI)

10.1186/1465-9921-9-54

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