Transcriptomic Analysis in the Mouse Brain
Author Information
Author(s): Charles Plessy, Michela Fagiolini, Akiko Wagatsuma, Norihiro Harasawa, Takenobu Kuji, Atsuko Asaka-Oba, Yukari Kanzaki, Sayaka Fujishima, Kazunori Waki, Hiroyuki Nakahara, Takao K. Hensch, Piero Carninci
Primary Institution: RIKEN
Hypothesis
Can we create a comprehensive resource for transcriptomic analysis in the mouse visual cortex?
Conclusion
The study provides an annotated cDNA clone collection that is useful for transcriptomic analysis in the mouse brain, revealing that over 30% of clones are differentially expressed.
Supporting Evidence
- The study created a collection of 16,209 RIKEN clones from mouse visual cortex.
- More than 30% of the clones were found to be differentially expressed.
- The cDNA collection is suitable for probing the transcriptome of purified cell populations.
Takeaway
The researchers made a collection of DNA pieces from mouse brains to help understand how different brain cells work. They found that many of these pieces are expressed differently in various cell types.
Methodology
The study involved preparing a low-redundancy set of cDNA clones and using microarrays to compare gene expression in different cell types from the mouse visual cortex.
Potential Biases
There may be biases in the selection of clones and the interpretation of differential expression due to the complexity of the transcriptome.
Limitations
The study may not account for all variations in gene expression due to the complexity of the transcriptome and potential noise from other cell types.
Participant Demographics
C57Bl/6 BAC transgenic mice expressing EGFP under the control of parvalbumin regulatory sequences were used.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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