How Dynactin Helps Adenovirus Move Inside Cells
Author Information
Author(s): Engelke Martin F., Burckhardt Christoph J., Morf Matthias K., Greber Urs F.
Primary Institution: Institute of Molecular Life Sciences, University of Zurich
Hypothesis
Does dynactin play a specific role in the cytoplasmic trafficking of adenovirus on microtubules?
Conclusion
Dynactin enhances adenovirus infection by increasing the speed and efficiency of dynein-mediated virus motion to the nucleus.
Supporting Evidence
- Overexpression of dynactin subunits reduced adenovirus infection.
- Live cell imaging showed distinct motion patterns of adenovirus.
- Microtubules were required for adenovirus infection.
Takeaway
Dynactin is like a helper that makes sure the adenovirus moves quickly to where it needs to go inside the cell, helping it infect the cell better.
Methodology
Live cell spinning disc confocal microscopy was used to track single virus particles and analyze their motion patterns.
Limitations
The study primarily focuses on one type of virus and may not generalize to all viruses.
Participant Demographics
HeLa cells were used for the experiments.
Statistical Information
P-Value
0.004
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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