HAPIscreen: A New Method for Identifying Aptamers
Author Information
Author(s): Dausse Eric, Taouji Saïd, Evadé Laetitia, Di Primo Carmelo, Chevet Eric, Toulmé Jean-Jacques
Primary Institution: Inserm U869, Institut Européen de Chimie et Biologie
Hypothesis
Can a new method improve the identification of high-affinity aptamers from large oligonucleotide libraries?
Conclusion
HAPIscreen is a faster and less biased method for identifying aptamers compared to traditional SELEX procedures.
Supporting Evidence
- HAPIscreen allows for the functional identification of high affinity aptamers without prior sequencing.
- The methodology can be adapted for automation and screening larger numbers of candidates.
- HAPIscreen demonstrated the ability to identify new RNA aptamers to pre-microRNAs.
Takeaway
HAPIscreen is a new way to find special molecules called aptamers that can stick to other molecules really well, and it does this faster than older methods.
Methodology
The study developed a homogeneous solution-based method using AlphaScreen® technology to screen large populations of oligonucleotide candidates generated from SELEX.
Potential Biases
The traditional SELEX process can mask low copy/high affinity aptamer candidates due to amplification bias.
Limitations
The method may still be biased if the fixed sequences used for capturing candidates interfere with their binding properties.
Digital Object Identifier (DOI)
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