Expression of 5S rDNA in the oocytes of water frogs
2009
Expression of 5S rDNA in Water Frog Oocytes
Sample size: 10
publication
Evidence: moderate
Author Information
Author(s): Czarniewska Elżbieta, Plewa Robert
Primary Institution: Adam Mickiewicz University
Hypothesis
What is the expression pattern of 5S rDNA in the oocytes of water frogs?
Conclusion
5S rDNA can be used as an internal control gene in studies of relative mRNA quantitative assays in water frog oocytes.
Supporting Evidence
- 5S rDNA expression in Rana ridibunda oocytes is approximately six times higher than in Rana lessonae oocytes.
- Rana esculenta oocytes show similar 5S rDNA expression levels to Rana ridibunda oocytes.
- The study suggests using 5S rDNA as a stable internal control gene for mRNA quantification in frog oocytes.
Takeaway
This study looked at how a specific gene is expressed in frog eggs, finding that one type of frog's eggs show much higher levels of this gene than another type.
Methodology
The study used quantitative real-time PCR to analyze the expression levels of 5S rDNA in oocytes from different frog species.
Participant Demographics
Oocytes from three species of water frogs: Rana lessonae, Rana ridibunda, and Rana esculenta.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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