MIER1-beta and its Role in Regulating CBP Activity
Author Information
Author(s): Tina Blackmore, Corinne F. Mercer, Gary D. Paterno, Laura L. Gillespie
Primary Institution: Terry Fox Cancer Research Laboratories, Division of BioMedical Sciences, Faculty of Medicine, Memorial University of Newfoundland
Hypothesis
How does MIER1 interact with and regulate the histone acetyltransferase activity of CBP?
Conclusion
MIER1 inhibits the histone acetyltransferase activity of CBP without interfering with its ability to bind histones.
Supporting Evidence
- MIER1 interacts with the C-terminal half of CBP, which includes important functional domains.
- When MIER1 is present, CBP shows no detectable histone acetyltransferase activity.
- The inhibition of CBP's activity by MIER1 is not due to interference with histone binding.
Takeaway
MIER1 is like a switch that turns off a machine (CBP) that helps add special tags (acetyl groups) to proteins, which is important for how genes work.
Methodology
The study used GST pull-down assays and HAT assays in HEK293 cells to investigate the interaction between MIER1 and CBP.
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website