Bovine Neutrophil Responses to Extracellular Vesicles from Besnoitia besnoiti
Author Information
Author(s): Espinosa Gabriel, Salinas-Varas Constanza, Rojas-Barón Lisbeth, Preußer Christian, Pogge von Strandmann Elke, Gärtner Ulrich, Conejeros Iván, Hermosilla Carlos, Taubert Anja
Primary Institution: Institute of Parasitology, Justus Liebig University Giessen, Giessen, Germany
Hypothesis
Does exposure to extracellular vesicles from Besnoitia besnoiti affect bovine polymorphonuclear neutrophil (PMN) functions?
Conclusion
Extracellular vesicles from B. besnoiti-infected cells can trigger neutrophil extracellular trap formation and the release of certain cytokines in bovine PMN.
Supporting Evidence
- EVs from B. besnoiti-infected BUVEC significantly induced extracellular DNA release.
- PMN stimulation with BUVEC- and tachyzoite-derived EVs confirmed NET formation.
- IL-1β and IL-6 release was significantly increased after PMN exposure to EVs from B. besnoiti-infected BUVEC.
Takeaway
This study shows that certain tiny particles released by infected cells can help immune cells in cows fight off a parasite.
Methodology
Bovine PMN were exposed to extracellular vesicles from different sources, and their responses were measured using various assays including NET formation and cytokine release.
Limitations
The study focused on immediate reactions of resting bovine PMN, which may not represent responses in activated states.
Participant Demographics
Healthy adult dairy cows were used as blood donors.
Statistical Information
P-Value
<0.001
Statistical Significance
p<0.001
Digital Object Identifier (DOI)
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