Study of P2X Receptors in Liver Cells and Their Response to HCV
Author Information
Author(s): Manzoor Sobia, Idrees Muhammad, Ashraf Javed, Mehmood Azra, Butt Sadia, Fatima Kaneez, Akbar Haji, Rehaman Irshad U, Qadri Ishtiaq
Primary Institution: National Centre of Excellence in Molecular Biology, University of the Punjab, Lahore, Pakistan
Hypothesis
The study aims to identify the presence of P2X receptors in human liver cells and their response to HCV structural proteins.
Conclusion
The study confirmed the presence of P2X receptors in human liver cells and showed that P2X4 is significantly responsive to HCV structural proteins.
Supporting Evidence
- Five different isoforms of P2X receptors were identified in Huh-7 cells.
- P2X4 receptor expression increased 6.2 fold in Huh-7/E1E2 cells compared to control.
- P2X2, P2X3, and P2X6 were unresponsive to HCV structural proteins.
Takeaway
This study found that certain receptors in liver cells react to a virus that causes liver disease, which could help us understand how the virus affects the liver.
Methodology
The study used human hepatoma cell line Huh-7 and its derivative Huh-7/E1E2 to analyze the expression of P2X receptors using RT-PCR and qRT-PCR.
Participant Demographics
The study involved human liver cells (Huh-7) derived from a hepatoma.
Statistical Information
P-Value
p ≤ 0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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