How PEPT-1 Transporter is Regulated in C. elegans
Author Information
Author(s): Benner Jacqueline, Daniel Hannelore, Spanier Britta
Primary Institution: ZIEL Research Center of Nutrition and Food Sciences, Technische Universität München, Freising, Germany
Hypothesis
The study investigates the modulators of the peptide transporter PEPT-1 in C. elegans and their effects on its function.
Conclusion
The study identifies four modulators that affect the function of the PEPT-1 transporter in C. elegans, suggesting a complex regulatory network involving amino acid sensing.
Supporting Evidence
- Gene silencing of four genes affected PEPT-1 function in C. elegans.
- Silencing of a glutathione peroxidase increased PEPT-1 transport function.
- Silencing of two cytosolic peptidases reduced PEPT-1 transport activity.
- Modulations of PEPT-1 function were conserved in human Caco-2/TC7 cells.
Takeaway
Scientists studied how a special transporter in tiny worms helps absorb proteins and found some genes that can change how well it works.
Methodology
The study used RNA interference to silence genes in C. elegans and measured the effects on PEPT-1 function through transport assays.
Limitations
The study primarily focuses on C. elegans, which may limit the generalizability of the findings to other organisms.
Statistical Information
P-Value
p<0.001
Statistical Significance
p<0.001
Digital Object Identifier (DOI)
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