Zyxin and SIRT1 Interaction in Neurons
Author Information
Author(s): Fujita Yuki, Yamaguchi Atsushi, Hata Katsuhiko, Endo Mitsuharu, Yamaguchi Naoto, Yamashita Toshihide
Primary Institution: Department of Molecular Neuroscience, Graduate School of Medicine, Osaka University
Hypothesis
Does zyxin interact with SIRT1 in the central nervous system?
Conclusion
Zyxin is a novel interacting partner of SIRT1, suggesting that SIRT1 may regulate signal transmission from the extracellular matrix to the nucleus by modulating zyxin's functions through deacetylation.
Supporting Evidence
- SIRT1 and zyxin are both expressed in the developing mouse brain.
- Zyxin accumulates in the nucleus and co-localizes with SIRT1 after treatment with leptomycin B.
- SIRT1 deacetylates zyxin, indicating a functional interaction.
Takeaway
Zyxin is a protein that helps cells communicate, and it works with another protein called SIRT1 to send signals from outside the cell to the inside.
Methodology
Yeast two-hybrid screening and co-immunoprecipitation assays were used to identify and confirm the interaction between SIRT1 and zyxin.
Limitations
The study does not explore the full biological significance of the interaction between SIRT1 and zyxin in various cellular contexts.
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website