Protein Expression Profiling in Developing Chick Retina
Author Information
Author(s): Finnegan Sorcha, Robson Joanne L, Wylie Mildred, Healy Adrienne, Stitt Alan W, Curry William J
Primary Institution: Queen's University of Belfast
Hypothesis
The study aims to employ proteomic analysis to study the developing chick retina throughout various stages of embryonic development.
Conclusion
The study adds a unique data set to previous proteomic investigations of retinal development, highlighting proteins important for normal retinal development in chicks.
Supporting Evidence
- The study identified 234 proteins with decreased expression and 198 proteins with increased expression from E12 to P33.
- Proteins such as alpha enolase and beta-synuclein showed significant changes in expression during retinal development.
- The research contributes to understanding the molecular events underpinning retinogenesis.
Takeaway
Scientists looked at proteins in baby chick eyes to understand how they grow and develop, which could help in treating eye diseases.
Methodology
Proteomic analysis using 2D gel electrophoresis and mass spectrometry to identify protein expression changes during retinal development.
Limitations
The study focused on a limited number of developmental stages and may not capture all relevant protein changes.
Participant Demographics
Fertilized White Leghorn chicken eggs were used for the study.
Statistical Information
P-Value
< 0.001
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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