Rapid non-genomic signalling by 17β-oestradiol through c-Src involves mTOR-dependent expression of HIF-1α in breast cancer cells
2011
How Oestradiol Affects HIF-1α in Breast Cancer Cells
publication
Evidence: moderate
Author Information
Author(s): Sudhagar S, Sathya S, Lakshmi B S
Primary Institution: Centre for Biotechnology, Anna University, Chennai, India
Hypothesis
Does oestradiol induce HIF-1α expression in breast cancer cells through non-genomic signaling pathways?
Conclusion
Oestradiol induces HIF-1α expression in breast cancer cells via the ERα/c-Src/PI3K/AKT/mTOR signaling pathway.
Supporting Evidence
- Oestradiol rapidly activates the signaling cascade leading to HIF-1α protein synthesis.
- The study shows that HIF-1α expression is regulated by the mTOR pathway in response to oestradiol.
- Pharmacological inhibitors of ERα, c-Src, PI3K, and mTOR block HIF-1α expression.
Takeaway
Oestradiol helps breast cancer cells make a protein called HIF-1α, which is important for their growth, by using a special signaling pathway.
Methodology
The study used western blot analysis and HIF1 reporter assays to measure HIF-1α expression and its signaling pathways.
Participant Demographics
The study focused on human breast cancer cell lines, specifically MCF-7, T47D, and MDA-MB-231.
Digital Object Identifier (DOI)
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