Chromatin Accessibility in Mouse Oocytes During Configuration Transition
Author Information
Author(s): Zhu Shuai, Li Jiashuo, Wang Xiuwan, Jin Yifei, Wang Hengjie, An Huiqing, Sun Hongzheng, Han Longsen, Shen Bin, Wang Qiang
Primary Institution: Nanjing Medical University
Hypothesis
The study investigates the chromatin accessibility landscape of mouse oocytes during the transition from non-surrounded nucleolus (NSN) to surrounded nucleolus (SN) and its implications for developmental competence.
Conclusion
The study provides insights into the chromatin dynamics and their relationship with histone modifications and transcriptional activity during oocyte development.
Supporting Evidence
- The study establishes a comprehensive resource for probing configuration transition in oocytes.
- Chromatin accessibility is closely associated with histone H3K4me3 during the transition.
- DNA methylation levels are higher in surrounded nucleolus oocytes compared to non-surrounded nucleolus oocytes.
- Transcriptional activity is significantly reduced during the chromatin remodelling process.
Takeaway
This study looks at how the structure of DNA in mouse eggs changes as they get ready to develop, which is important for making healthy babies.
Methodology
The study utilized multi-omics approaches including ATAC-seq, BS-seq, and CUT&Tag to analyze chromatin accessibility, DNA methylation, and histone modifications in mouse oocytes.
Limitations
The study primarily focuses on mouse oocytes, which may limit the generalizability of the findings to other species.
Participant Demographics
Three-week-old female C57BL/6J mice were used in the experiments.
Statistical Information
P-Value
p<0.001
Statistical Significance
p<0.001
Digital Object Identifier (DOI)
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