Effects of Omega-3 and Omega-6 Fatty Acids on Prostaglandin E Secretion in Fat Cells
Author Information
Author(s): Wortman Patrick, Miyazaki Yuko, Kalupahana Nishan S, Kim Suyeon, Hansen-Petrik Melissa, Saxton Arnold M, Claycombe Kate J, Voy Brynn H, Whelan Jay, Moustaid-Moussa Naima
Primary Institution: University of Tennessee (UT), Department of Animal Science, Knoxville, TN, USA
Hypothesis
Do eicosapentaenoic acid (EPA) and arachidonic acid (AA) differentially affect prostaglandin E2 (PGE2) secretion and fatty acid synthase (FAS) activity in adipocytes?
Conclusion
Both EPA and AA increased PGE2 secretion but did not significantly alter FAS activity, while COX-2 inhibition reduced both PGE2 production and FAS activity.
Supporting Evidence
- Both EPA and AA increased PGE2 secretion from adipocytes.
- COX-2 inhibition significantly decreased PGE2 secretion.
- Neither EPA nor AA significantly altered FAS activity despite increased PGE2 levels.
- FAS mRNA levels were significantly decreased by both EPA and AA treatments.
Takeaway
This study looked at how two types of fats, EPA and AA, affect a substance called PGE2 in fat cells. They found that both fats increased PGE2 but didn't change how much fat the cells made.
Methodology
The study used 3T3-L1 adipocytes to measure PGE2 secretion and FAS activity after treatment with EPA, AA, and a COX-2 inhibitor.
Potential Biases
Potential bias due to the use of a single cell line (3T3-L1) for experiments.
Limitations
The study did not measure the long-term effects of fatty acid treatments on adipocyte function.
Participant Demographics
Male C57BL/6J ApcMin/+ mice were used for in vivo experiments.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website