Serotype and genotype analysis of dengue virus by sequencing followed by phylogenetic analysis using samples from three mini outbreaks-2007-2009 in Pakistan
2011

Analysis of Dengue Virus Outbreaks in Pakistan

Sample size: 114 publication Evidence: moderate

Author Information

Author(s): Fatima Zareen, Idrees Muhammad, Bajwa Mohammad A, Tahir Zarfishan, Ullah Obaid, Zia Muhammad Q, Hussain Abrar, Akram Madiha, Khubaib Bushra, Afzal Samia, Munir Saira, Saleem Sana, Rauff Bisma, Badar Sadaf, Naudhani Mahrukh, Butt Sadia, Aftab Mahwish, Ali Liaqat, Ali Muhammad

Primary Institution: Division of Molecular Virology, CEMB University of the Punjab

Hypothesis

What are the circulating serotypes of dengue virus in Pakistan during the outbreaks of 2007-2009?

Conclusion

The study found that serotype DEN-2 was the dominant strain of dengue virus in Pakistan during the analyzed outbreaks.

Supporting Evidence

  • A total of 114 serum samples were analyzed over three years.
  • 20 patients were found to be infected with dengue virus.
  • Serotype DEN-2 was found to be the most prevalent strain during the outbreaks.

Takeaway

Researchers looked at blood samples from people who got sick during dengue outbreaks in Pakistan and found that most had a type of the virus called DEN-2.

Methodology

The study used PCR and nucleotide sequencing to analyze serum samples collected from patients during dengue outbreaks.

Limitations

The study had a small number of sequenced samples, which may not represent the full diversity of dengue virus strains.

Digital Object Identifier (DOI)

10.1186/1471-2180-11-200

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