Detection of Streptococcus mutans in Human Saliva and Blood Samples
Author Information
Author(s): Alexandre R. Vieira, Kathleen B. Deeley, Nicholas F. Callahan, Jacqueline B. Noel, Ida Anjomshoaa, Wendy M. Carricato, Louise P. Schulhof, Rebecca S. DeSensi, Pooja Gandhi, Judith M. Resick, Carla A. Brandon, Christopher Rozhon, Asli Patir, Mine Yildirim, Fernando A. Poletta, Juan C. Mereb, Ariadne Letra, Renato Menezes, Steven Wendell, Jorge S. Lopez-Camelo, Eduardo E. Castilla, Iêda M. Orioli, Figen Seymen, Robert J. Weyant, Richard Crout, Daniel W. McNeil, Adriana Modesto, Mary L. Marazita
Primary Institution: University of Pittsburgh
Hypothesis
Can genomic DNA copies of Streptococcus mutans be detected in human DNA samples extracted from blood?
Conclusion
The study found that while genomic DNA copies of Streptococcus mutans can be detected in saliva from both caries-free and caries-affected individuals, they could not be detected in blood samples.
Supporting Evidence
- Streptococcus mutans was detected in saliva samples from both caries-free and caries-affected individuals.
- Genomic DNA copies of Streptococcus mutans were not detected in blood samples.
- Correlation between threshold cycle values and caries experience was significant only in children.
Takeaway
The researchers looked for a germ that causes cavities in people's spit and blood, but they only found it in spit, not blood.
Methodology
Real-time PCR was used to detect genomic DNA from Streptococcus mutans in saliva and blood samples.
Limitations
The assay may not be sensitive enough to detect Streptococcus mutans in blood samples.
Participant Demographics
Participants included individuals aged 1 to 89 years, with a demographic distribution reflective of the city of Pittsburgh, and various socioeconomic backgrounds.
Statistical Information
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website