Development of a multi-locus sequence typing scheme for Laribacter hongkongensis, a novel bacterium associated with freshwater fish-borne gastroenteritis and traveler's diarrhea
2009

New Typing System for Laribacter hongkongensis

Sample size: 146 publication Evidence: high

Author Information

Author(s): Woo Patrick CY, Teng Jade LL, Tsang Alan KL, Tse Herman, Tsang Vivien YM, Chan King-Man, Lee Edwin KY, Chan Jim KH, Ma Shirley SL, Tam Dorothy MW, Chung Liliane MW, Lau Susanna KP, Yuen Kwok-Yung

Primary Institution: The University of Hong Kong

Hypothesis

A highly reproducible and discriminative typing system is essential for better understanding of the epidemiology of L. hongkongensis.

Conclusion

A highly reproducible and discriminative MLST system was developed for L. hongkongensis.

Supporting Evidence

  • The MLST system was used to characterize 146 L. hongkongensis isolates.
  • A total of 97 different sequence types (STs) were assigned to the 146 isolates.
  • The overall discriminatory power was 0.9861.

Takeaway

Scientists created a new way to identify a germ called Laribacter hongkongensis that can make people sick from fish. This helps understand how it spreads.

Methodology

The study involved developing a multilocus sequence typing (MLST) system using 146 isolates from humans and fish, analyzing seven housekeeping genes.

Participant Demographics

39 isolates from humans and 107 isolates from fish.

Statistical Information

P-Value

P = 0

Statistical Significance

p<0.05

Digital Object Identifier (DOI)

10.1186/1471-2180-9-21

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