BAC Library Construction for Culex quinquefasciatus
Author Information
Author(s): Hickner Paul V, deBruyn Becky, Lovin Diane D, Mori Akio, Saski Christopher A, Severson David W
Primary Institution: University of Notre Dame
Hypothesis
The construction of a BAC library will enhance genome investigations in Culex quinquefasciatus.
Conclusion
The NDJ library represents an excellent resource for genome assembly enhancement and characterization in Culex pipiens complex mosquitoes.
Supporting Evidence
- The NDJ library consists of 144 384-well microplates containing 55,296 clones.
- Fragment insert size ranged from 50 to 190 kb in length with a mean of 106 kb.
- The BAC library provides approximately 10.1-fold coverage of the Cx. quinquefasciatus genome.
- PCR screening resulted in a mean of 9.0 positive plate pools per locus.
Takeaway
Scientists built a special library of DNA from a mosquito to help understand its genes better.
Methodology
A BAC library was constructed using high molecular weight DNA extracted from Culex quinquefasciatus pupae and partially digested with HindIII.
Limitations
The genome assembly remains highly fragmented and few supercontigs have been assigned to their respective chromosomes.
Digital Object Identifier (DOI)
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