Rapid Detection of Asia1 Foot-and-Mouth Disease Virus Using Colloidal Gold Strips
Author Information
Author(s): Lin Tong, Shao Jun-jun, Du Jun-zheng, Cong Guo-zheng, Gao Shan-dian, Chang Huiyun
Primary Institution: State Key Laboratory of Veterinary Etiological Biology, National Foot and Mouth Disease Reference Laboratory, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences
Hypothesis
Can a colloidal gold strip be developed for the rapid detection of type Asia1 foot-and-mouth disease virus?
Conclusion
The developed colloidal gold strips provide a rapid and effective method for detecting type Asia1 FMDV without the need for specialized equipment.
Supporting Evidence
- The detection sensitivity was found to be as high as 10-5 dilution of Asia1/JSL/05.
- The agreement between the results obtained by CGS and reverse indirect hemagglutination assay reached 98.75%.
- The developed strip showed no cross-reaction with other types of FMD and SVDV.
Takeaway
Scientists created a quick test that can tell if animals have a specific virus that makes them sick, and it works really well.
Methodology
The study involved creating monoclonal antibodies and using them in a colloidal gold strip test to detect the Asia1 FMDV.
Potential Biases
Potential bias in the specificity and sensitivity results due to the limited range of tested samples.
Limitations
The study primarily focused on type Asia1 and may not be applicable to other serotypes of FMDV.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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