Deletion of ultraconserved elements yields viable mice
2007

Viable Mice Without Ultraconserved Elements

publication Evidence: moderate

Author Information

Author(s): Ahituv Nadav, Zhu Yiwen, Visel Axel, Holt Amy, Afzal Veena, Pennacchio Len A, Rubin Edward M

Primary Institution: Lawrence Berkeley National Laboratory

Hypothesis

Are ultraconserved genetic elements really indispensable?

Conclusion

Mice lacking ultraconserved elements are viable and show no significant abnormalities.

Supporting Evidence

  • All four lines of mice lacking ultraconserved elements were viable and fertile.
  • No critical abnormalities were observed in various phenotypes including growth and metabolism.
  • The results suggest that extreme sequence conservation does not necessarily indicate essential functions.

Takeaway

Scientists removed some important-looking DNA from mice, and surprisingly, the mice were still healthy and had babies.

Methodology

Four noncoding ultraconserved elements were deleted from the mouse genome using genetic engineering techniques.

Limitations

The study may not have detected all possible phenotypic impacts of the deleted sequences.

Statistical Information

P-Value

0.64

Statistical Significance

p=0.64

Digital Object Identifier (DOI)

10.1371/journal.pbio.0050234

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